By Henrik Nielsen (auth.), Henrik Nielsen (eds.)

Recent perception into the transcripts generated from the mammalian genome (i.e. the transcriptome) has printed that transcription is a much more advanced phenomenon than formerly notion. In RNA: equipment and Protocols, specialist researchers give you the techniques and strategies used to explain the constitution of messenger RNAs and non-coding RNAs which are transcribed through RNA polymerase II because the speedy gene items in mammalian cells. interested by the constitution of the RNA items of “gene X” and the mapping of proteins linked to those RNAs, the quantity offers applicable details for non-specialists in RNA biology. Written within the hugely profitable equipment in Molecular Biology™ sequence structure, many chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and key tips about troubleshooting and warding off identified pitfalls. accomplished and useful, RNA: tools and Protocols perspectives the transcriptional panorama with an appreciation for the function that proteins play within the processing and interpretation of genetic info in an try to extra our the most important wisdom of the various items and complex regulatory networks that end result from it.

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