By Christiane Guguen-Guillouzo, Andre Guillouzo (auth.), Patrick Maurel (eds.)
Hepatocytes account for about eighty% of the liver mass and play an important position in quite a few elements of liver physiopathology, displaying unmatched complexity and variety of services. In Hepatocytes: equipment and Protocols, specialist researchers give you the reader with tools, technical protocols, and evaluation chapters targeting chosen parts of hepatocyte biology together with isolation, tradition, differentiation and stem cells, and hepatocyte use in scientific, simple, and utilized learn. With a selected emphasis on human hepatocytes, the quantity provides chapters overlaying topics together with hepatocyte tradition versions, cryopreservation tools, differentiation review, liver ontogenesis, construction of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and shipping, bile acid and blood coagulation issue creation, an infection by means of HBV and HCV, humanized animals, biortificial liver units, hepatocyte transplantation. As a quantity within the hugely profitable Methods in Molecular Biology™ sequence, protocol chapters comprise short introductions to their respective issues, lists of the required fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, and notes on troubleshooting and keeping off recognized pitfalls.
Comprehensive and state-of-the-art, Hepatocytes: equipment and Protocols can be important to all those who find themselves at present utilizing or making plans to take advantage of human, or animal, hepatocytes to enquire any point of liver physiopathology or who're attracted to liver improvement or liver stem cells and liver biotherapy.
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