By John J. Bozzola (auth.), John Kuo (eds.)

The revised and multiplied moment variation of this booklet offers the latest expertise in electron microscopy, whereas preserving the practicality and accessibility of the acclaimed first variation. just like the first version, this quantity presents transparent, concise directions on processing organic specimens and contains dialogue at the underlying rules of the vast majority of the techniques offered. Electron Microscopy includes significant components of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM region covers a number of key strategies, together with: traditional specimen practise tools for classy cells and biomedical and plant tissues; cryospecimen coaching by way of high-pressure freezing and cryoultramicrotomy destructive staining and immunogold labeling ideas; and TEM crystallography and cryo-TEM tomography. The SEM region equally attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy thoughts, in addition to the appliance of X-ray microanalysis. Protocols for the applying of X-ray microanalysis to SEM and mass spectrometry finish the volume.

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